Microbes are essential for the functioning of life on earth, yet a lack of awareness of their positive activities persists in society. In the UK, microbiology is scarcely taught before secondary education. Therefore, we organised 'Science Fun Days' for primary school pupils (aged 9-11 years) in 2024 and 2025, with the aims of increasing their microbiological awareness and, more generally, promoting positive attitudes towards science and nature. Over 450 pupils attended a Science Fun Day hosted at the University of Essex, which involved hands-on activities in the laboratory and outdoors. Pre-event and post-event surveys were completed by 307 and 305 of these pupils, respectively, from across seven schools. The surveys revealed that, after participating in a Science Fun Day, the proportion of pupils who would like a job in science increased from 29.6% to 41.9% in 2024 and 21.8% to 32.9% in 2025. Pupils from schools located in areas of high deprivation rated their desire for a science career significantly higher overall than pupils from schools located in low deprivation areas. Surveys also captured a post-event increase in the percentage of pupils that know what microbes are from 68.7% to 88.0% in 2024 and 49.3% to 79.1% in 2025. Gender differences were minimal and included higher overall perceived confidence in science lessons by male-identifying students; however, female-identifying students reported similar levels of confidence as their male-identifying peers in the post-event survey. Our results support the value of extra-curricular excursions to boost children's understanding of microbiology, enable positive attitudes towards science, and encourage science-related career aspirations.